Abstract
In the present study, mitochondrial DNA sequences from the cytochrome b (cytb) gene and seven nuclear microsatellites were examined to assess the genetic diversity of Epinephelus marginatus inhabiting Tunisian and Libyan coastal waters. Based on 940 base pairs of the cytochrome b segment, we found low level of genetic variability for the two samples analysed (h = 0.294 ± 0.097and h = 0.274± 0.142 respectively in Tunisia and Libya). An analysis of molecular variance (AMOVA) showed significant pattern of genetic structure based on nuclear data (7 microsatellite loci) (ΦST= 0.28487; P < 0.001). Conversely, no genetic structuring was found for mtDNA (ΦST = -0.0121). In summary, this study provides preliminary assessment of geographical patterns of differentiation of E. marginatus in this region for conservation, management and stock identification.
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