Population genetic structure and selective pressure on the mitochondrial ATP6 gene of the Japanese sand lance Ammodytes personatus Girard

Abstract

AbstractThermoregulation has been suggested to influence mitochondrial DNA (mtDNA) evolution. Previous studies revealed that the mitochondrial protein-coding genes of fish living in temperate climates have smallerdN/dS(Non-synonymous substitution rate/Synonymous substitution rate) than tropical species. However, it is unknown whether different geographic populations of one fish species experience stronger selective pressures between cold and warm climates. The biological characteristics of the Japanese sand lance,Ammodytes personatusin the North-western Pacific is well-suited for assessing the performance of mtDNA evolution among separate geographic populations. In this study, we focused on the mitochondrial ATP6 gene ofA. personatususing 174 individuals from eight different sea temperature populations. Two distinct haplotype lineages and a significant population structure (P= 0.016) were found in this species. The frequencies of the two lineages varied with the changes of annual sea temperature. The southern lineage (lineage A,dN/dS= 0.0384) showed a largerdN/dSvalue than the northern lineage (lineage B,dN/dS= 0.0167), suggesting that sea temperature greatly influences the evolution of the two lineages. The result provides robust evidence of local adaptation between populations inA. personatus.