Population dynamics of Diaphorina citri with the implementation of integrated management of healthy orange gardens (PTKJS) and CVPD detection with PCR engineering

Abstract

This study was to determine the population dynamics of Diaphorina citri with the Application of Integrated Management of Healthy Citrus Gardens (PTKJS) and detection of CVPD by PCR technique. This research was conducted in a village in Tebing Batu village, Sebawi District, Sambas Regency from February to October 2018. The research used observation / monitoring techniques for insect vectors, the abundance and diversity of insects and disease symptoms using the PCR technique. The results showed that the application of monitoring techniques which was continued by PTKJS, there was an increase in crop yields and an increase in the diversity of the highest insect species trapped after the implementation of PTKJS was dominated by the Hymenoptera Order with 17 families out of a total of 7515 trapped insects. The application of monitoring techniques has resulted in an average emphasis of Diaphorina citri from 16,33 to 1,67. The PCR method is a very sensitive method and can be used to detect pathogenic bacteria that cause CVPD based on testing of 27 samples repeated 3 times showing> 90% positive indicating the presence of L. asiaticum bacterial infection that causes CVPD.