Population data for 38 autosomal insertion/deletion (InDels) and 50 SNPS polymorphisms in Argentinean population

Abstract

Forensic laboratories must add as much genetic information as possible in order to solve the broadened variety of post-mortem paternity and kinship cases now routinely presented. Often, use of STR autosomal markers alone is not enough to reach a high discrimination power, especially when dealing with degraded post-mortem material or complex family pedigrees. In order to expand the set of genetic markers used to solve challenging cases, and since reference databases are required in forensic application, allele frequencies and forensic parameters for 38 InDel and 50 SNP binary markers were calculated from a sample of thirty (for SNPs) and fifty (for InDels) unrelated individuals from the two most populous cities of Argentina: Córdoba and Buenos Aires. DNA was extracted with a modified salting out procedure and amplification products were detected on an ABI 3130 Genetic Analyzer (Applied Biosystems). No significant deviations from Hardy–Weinberg expectations were found. In the analyzed sample, no pairwise linkage disequilibrium between InDels and SNPs was found neither among the 88 binary markers nor the 17 STR markers from commercial kits. Comparisons were also made with previously studied populations. The present database will be useful for forensic and paternity purposes for the above mentioned cities of Argentina. Moreover, these additional markers can help forensic laboratories to solve parentage testing as well as to improve the analysis of degraded DNA samples.