Abstract
Present report describes the immunohistochemical diagnosis of poorly differentiated squamous cell carcinoma and involvement of papilloma virus in a mare. A five years old mare was brought to the Teaching Veterinary Clinical Complex with history of multi-lobulated haemorrhagic ulcerative mass in the vulvar region since last two months. The swollen region was aseptically prepared and the mass was resected out under local anaesthesia. Cytology of tumour revealed large cells with abundant basophilic cytoplasm and pleomorphic nuclei. Microscopically, presence of irregular cords of undifferentiated epidermal cells proliferating in downward fashion and invading dermis and subcutis were seen. AgNOR staining of section revealed 4 to 5 irregular, large dots or bizarre clusters in nuclei indicating malignancy. The PCR amplification was done by using specific primers for PVL1 gene of CPV that amplified a 480 base pair fragment of the Papilloma Virus L1 gene. Pancytokeratin immunolabeling was helpful in the diagnosing poorly differentiated squamous cell carcinoma. PCR confirmation suggested role of papilloma virus in induction and progression of squamous cell carcinoma.
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