Pontibacter locisalis Sy30T sp. nov. isolated from soil collected from an abandoned saltern.

Abstract

A novel Gram-stain negative, red, rod-shaped, non-motile and aerobic bacterial strain, designated Sy30(T), was isolated from dry soils of an abandoned marine saltern at Weihai, China. 16S rRNA sequence analysis indicated that strain Sy30(T) belongs to the genus Pontibacter in the family Cytophagaceae, with sequence similarities ranging from 93.3 to 96.4 % with other type species of the genus Pontibacter. The predominant cellular fatty acids were iso-C15:0 and summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B). The major menaquinone was MK-7. The major polyamine was sym-homospermidine. The DNA G+C content was 47.7 mol%. The major polar lipids consisted of phosphatidylethanolamine, phosphoaminolipid and two unidentified polar lipid. Based on phenotypic, chemotaxonomic and phylogenetic analysis, strain Sy30(T) represents a novel species of the genus Pontibacter in the family Cytophagaceae, phylum Bacteroidetes, for which the name Pontibacter locisalis sp. nov. is proposed. The type strain is Sy30(T) (=KCTC 42498(T) = CICC AB 2015060(T)).