Polyvinylalcohol-carbazate (PVAC) reduces red blood cell hemolysis.

Felix Sellberg,Bo Nilsson,Jaan Hong,Folke Knutson,Tim Melander Bowden,Thomas Engstrand,David Berglund,Fanny Fredriksson

doi:10.1371/journal.pone.0225777

Abstract

The objective of this study was to investigate whether a soluble polymer and aldehyde-scavenger, polyvinylalcohol-carbazate (PVAC), can inhibit hemolysis in the storage of red blood cells (RBC). The effect of PVAC was assessed over a wide range of concentrations, using absorption spectroscopy to evaluate the level of hemolysis. Moreover, osmotic stability and aldehyde-scavenging potential of RBC were assessed after storage in PVAC. After test tube storage for two weeks, red blood cell hemolysis was lower with PVAC compared to controls (mean difference 23%, 95% CI 16-29%, p < 0.001). A higher level of hemolysis led to a pronounced effect with PVAC. RBC stored in PVAC improved both the binding of free aldehydes (p <0.001) and the osmotic stability (p = 0.0036). Erythrocytes stored with PVAC showed less hemolysis, which might be explained by the ability of PVACs to stabilize the cell membrane and decrease oxidative injury.

Highlights

Hemolysis is in most areas of medical science unwanted and it can be divided into hemolysis in vivo or in vitro.Hemolysis in vivo occurs in a variety of diseases and can have both intrinsic and extrinsic causes
After test tube storage for two weeks, red blood cell hemolysis was lower with PVAC compared to controls
Erythrocytes stored with PVAC showed less hemolysis, which might be explained by the ability of PVACs to stabilize the cell membrane and decrease oxidative injury

Summary

Introduction

Hemolysis is in most areas of medical science unwanted and it can be divided into hemolysis in vivo or in vitro. Hemolysis in vivo occurs in a variety of diseases and can have both intrinsic and extrinsic causes. Intrinsic causes are either structural or functional defects in the red blood cells (RBCs). The objective of this study was to investigate whether a soluble polymer and aldehyde-scavenger, polyvinylalcohol-carbazate (PVAC), can inhibit hemolysis in the storage of red blood cells (RBC)

Objectives

Methods

Results

Conclusion