Polystyrene Immobilized Sol-Gel Ground Silica Monolith Particles Using One-Pot Reaction of Enhanced Separation Efficiency.

Abstract

The stationary phase based on sol-gel ground silica monolith particles has been produced by one-pot polymerization method incorporation of styrene and ethylene dimethacrylate. First, the ground silica monolith particles were prepared by a sol-gel process followed by sedimentation. The particles were then subjected to modify with styrene ligand via one-pot polymerization, whereas ethylene dimethacrylate was used as the cross-linker. The glass lined stainless steel columns (1mm internal diameter, 150mm length) were packed with the above phase for estimation of the chromatographic performance in high-performance liquid chromatography. An average number of theoretical plates of as high as 39,300 plates/column was obtained under the optimized elution condition. The column-to-column reproducibility was proved satisfactory in separation efficiency and retention factor. The experimental results indicate that sol-gel ground silica particles prepared by an aid of one-pot modification can provide a better way for preparation of highly efficient stationary phase.