Abstract
One of the most abundant pigments in certain recent sediments (Black Sea, Mediterranean Sea, Peru margin, Florida Bay) is an unstable derivative of chlorophyll‐a known as 132, 173‐cyclopheophorbide a‐enol (aka CPP, CYCLO, CPP516). To date, the purification of synthetic or natural samples of this compound has always been challenging, since it is readily converted to a ∼1:1 mixture of (132 R/S) chlorophyllones a (Chlone), 151‐ hydroxychlorophyllonelactone‐a, and/or other derivatives when chromatographed over silica gel or alumina supports in normal or reversed phase modes. In this study, Polystyrene‐Divinylbenzene (PS‐DVB) support (aka Polymeric Reversed Phase, or PRP) is introduced as an efficient stationary phase for the flash column chromatographic purification of CPP with little oxidation/conversion to artifacts. Similarly, PRP‐1 in analytical sized columns is shown to be highly useful for purity verification prior to UV/Vis, FTIR, and NMR characterizations.
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