Abstract
Modified polystyrene with Pt(IV)–azomethine (APS–Sch–Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS–Sch–Pt–GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)–azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS–Sch–Pt–GOx), increases the enzymes’ activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.
Highlights
Enzymes are used as biocatalysts in the chemical, pharmaceutical and food industries and as specific ligands in clinical and chemical analyses [1]
Studying (APS–Sch–Pt), two maximum activities were observed at phosphate buffer (pH) 4.0 and pH 7.0, which is different from earlier reports [14–16]
Our results indicate that the covalent binding of glucose oxidase enzyme (GOx) using Pt(IV)–Schiff base helped the enzyme to retain catalytic activity when it was repeatedly used in various cycles
Summary
Enzymes are used as biocatalysts in the chemical, pharmaceutical and food industries and as specific ligands in clinical and chemical analyses [1]. One of the most useful immobilization methods is covalent attachment of the enzyme onto a solid support. In the one point covalent attachment, only one (or two) functional groups of the enzyme is (are) connected to the polymer support via covalent binding. Hydroxyl groups can act as good chelating agents with metal ions as coordination covalent bonds. To investigate to what extent the effect of coordination covalent bonds depends on enzyme immobilization, we prepared a polymeric support involving Pt(IV) ions. To prepare such a polymeric support, the (aminomethyl) polystyrene (APS) reacted with 2-hydroxybenzaldehyde (APS–Sch; Scheme 1A) and the metal-containing complex was prepared using PtCl4 salts (APS–Sch–Pt),. Synthesis route of support for immobilization of glucose oxidase enzyme (GOx) enzyme
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