Abstract
The presence of polymeric actin in the polysome preparations from broad bean leaves (Vicia faba L. var. Russkie chernye) was detected on dot blots and under the electron microscope using the phalloidin-colloidal gold marker. The fractions enriched in the cytoskeleton-bound polysomes were isolated using different sensitivity of the membranes and cytoskeletal elements to nonionic and ionic detergents. The distribution of a polypeptide with a molecular weight of about 40 kDa in the subcellular fractions was studied and an enrichment of this polypeptide was observed in membrane-bound and especially cytoskeletal-bound polysomes. This polypeptide gave a positive signal when probed with anti-actin serum on Western blots.
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