Abstract
The contents of Deiters neurons isolated from the lateral vestibular nucleus of the rabbit were emptied into microdroplets by a free-hand dissection technique, which made it possible to avoid contamination from glia cell contents. Electron microscopic examination of shadowed and of stained specimens of the contents of the droplets revealed a large number of 230 Å ribosomes as well as smaller particles having a diameter of about 100 Å. A large proportion of both these classes of particles were found in discrete polysomal formations. The majority of the ribosomal polysomes were composed of 4 or 5 ribosomes, but larger, well defined polysomes containing up to 15 ribosomes were observed. A thin filament was sometimes found between the polysomal ribosomes. In addition, huge clusters containing up to a hundred ribosomes were encountered. Treatment of the nerve cell contents with ribonuclease was followed by disappearance of the polysomal organization. Stimulation of the RNA and protein synthesis by in vivo administration of tricyanoaminopropene did not significantly alter the pattern of the polysomes.
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