Abstract
The main purpose of this study was the preparation, optimization, characterization, and in vitro immunological activity evaluation of the polysaccharides of Atractylodes macrocephala Koidz-loaded nanostructured lipid carriers (PAMK-NLC). Both the blank NLC and PAMK-NLC were prepared using melt-emulsification and ultra-sonication techniques. The optimization of preparation conditions were a stearic acid to caprylic/capric triglyceride mass ratio of 2:1, a poloxamer 188 to soybean lecithin mass ratio of 2:1, and an ultrasound time of 12 min; these parameters were identified using response surface methodology (RSM). Under these conditions, the encapsulation efficiency was around 76.85% and the polydispersity index was around 0.281, indicating a good dimensional distribution. Furthermore, the potential of PAMK-NLC to enhance immunological activity in vitro was investigated in murine splenic lymphocytes. The results showed that PAMK-NLC could significantly stimulate lymphocyte proliferation and increase the ratio of CD4+ to CD8+ T cells. In conclusion, these findings indicated that PAMK-NLC possessed an efficient immunological activity, and that NLC is suitable for the delivery and protection of PAMK in clinical use.
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