Polysaccharides isolated from &lt;i&gt;Morinda officinalis&lt;/i&gt; How roots inhibits cyclophosphamide-induced leukopenia in mice

Yaxin Zhao,Meng Wang,Yang Li,Wanting Dong

doi:10.4314/tjpr.v16i9.16

Abstract

Purpose: To investigate the optimum parameters for extracting polysaccharides from Morinda officinalis How (MOP), and explore their inhibitory effects on leukopenia in mice. Methods: Orthogonal design was performed to investigate the optimum parameters for extracting MOP. A leukopenia mouse model was established by injection of cyclophosphamide (CTX) for three days. Thereafter, MOP (100, 200 and 400 mg/kg) was administered orally for 10 days. Furthermore, blood cells (leukocytes, neutrophil, lymphocyte and mononuclear cell) were analyzed, while serum IL-3 and IL- 6 were determined by ELISA. The thymus and spleen of the mice were separated and weighed to determine viscera indices. Results: Orthogonal design showed that the influence order of the four factors was extraction times (C) > ratio of water to raw material (RWM, D) > extraction time (B) > extraction temperature (A). The optimum extraction parameters for MOP were: extraction temperature (80 °C), extraction duration (2 h), no. of extractions (3), and ratio of water to raw material (30 mL/g). Furthermore, the results indicate that MOP (100, 200 and 400 mg/kg) elevated the levels of leukocyte ( p < 0.01), neutrophil ( p < 0.01), lymphocyte ( p < 0.01) and mononuclear cell ( p < 0.01) in leukopenia mice. Besides, MOP (100, 200 and 400 mg/kg) also increased thymus ( p < 0.01) and spleen ( p < 0.05) indices and serum levels of IL-3 ( p < 0.05) and IL-6 ( p < 0.01). Conclusion: Orthogonal design is a good strategy for optimizing extraction parameters of MOP. Furthermore, MOP stimulated synthesis of leukocytes in CTX-induced leukopenia in mice. Thus, MOP is a potential adjunct for the treatment of tumors/cancers. Keywords: Morinda officinalis , Polyscacharide, Orthogonal design, Leukopenia, Thymus index, Spleen index

Highlights

In recent years, studies have demonstrated that malignant cancers have become one of the most common incurable diseases worldwide [1]
Based on the range analysis results, ANOVA was determined, and the results showed that the extraction times and RWM were significant (F < 0.05) (Table 3)
No obvious significance was observed in the extraction temperature (A) and extraction time (B) (F > 0.05)

Summary

Introduction

Studies have demonstrated that malignant cancers have become one of the most common incurable diseases worldwide [1]. For the treatment of cancers, besides surgery, longterm chemotherapy is another commonly used effective method [2]. Chemotherapy could result in myelosuppression, leading to many serious side-effects, especially leukopenia [3]. Leukopenia could result in extremely low leukocyte levels in the body, causing hypoimmunity. In this situation, severe inflammatory cascades reactions could be induced, leading to sepsis and even death [4]. It is important to prevent leukopenia in the process of chemotherapy for treating cancers

Results

Discussion

Conclusion