Abstract
► Determine extraction conditions of polysaccharide from Laminaria japonica (LP). ► Reveal the method to remove proteins from LP. ► Determine the precipitation condition of LP by ethanol. ► Investigate physicochemical properties of LP1, LP2 and LP3 fractionated from LP. ► Evaluate the hypolipidemic activity of crude LP. The crude polysaccharides of Laminaria japonica (LP) were extracted with the optimal conditions as follows: raw materials to water 1:50 (w/v), extraction temperature 60 °C and extraction time 60 min. Trypsinase (6 U/ml) in combination with sevag reagent was beneficial to the removal of proteins from crude LP. Three fractions of LP1, LP2 and LP3 were obtained from the crude LP by using a final concentration of 40%, 60% and 80% ethanol to precipitate in turn. The three fractions showed obvious differences in monosaccharide compositions, molecular weight distribution, viscosity, IR spectroscopy and glycosyl linkages. In comparison with the control group, crude LP at a dose of 400 mg/kg/day caused a reduction in total serum cholesterol, triglycerides, high density lipoprotein-cholesterol and low density lipoprotein-cholesterol in serum by 80.6%, 63.4%, 43.8% and 79.8%, respectively. Moreover, crude LP exhibited good potential of enhancing antioxidant enzyme activities in serum of atherosclerosis mice.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
