Abstract
The polysaccharidic fractions isolated from Hemileia vastatrix uredospores by alkali treatment, expressed as a percentage of the initial uredospore weight, gave the following yields: 1 M NaOH soluble at 22°C (7.1); 1 M NaOH soluble at 60°C (5.0); and insoluble residue (7.6). Both alkali-soluble fractions contained mannose and glucose as the major constituents, with glycosidic linkages of the β-1 → 4 and β-1 → 3 types. The alkaliinsoluble residue contained predominantly glucosamine, and had infrared and X-ray spectra indistingushable from those of crustacean chitin. Electron microscope observations revealed that the insoluble residue consisted of the cell wall spines connected by a thin layer of microfibrils.
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