Abstract
The increasing need for point-of-care diagnosis has sparked the development of label-free sensing platforms, some of which are based on impedance measurements with biological cells. Here, interdigitated electrodes were functionalized with layer-by-layer (LbL) films of hyaluronan (HA) and chitosan (CHI) to detect prostatic tumor cells (PC3 line). The deposition of LbL films was confirmed with atomic force microscopy and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), which featured the vibrational modes of the HA top layer capable of interacting specifically with glycoprotein CD44 receptors overexpressed in tumor cells. Though the CHI/HA LbL films cannot be considered as a traditional biosensor due to their limited selectivity, it was possible to distinguish prostate tumor cells in the range from 50 to 600 cells/µL in in vitro experiments with impedance spectroscopy. This was achieved by treating the impedance data with information visualization methods, which confirmed the distinguishing ability of the films by observing the absence of false positives in a series of control experiments. The CD44–HA interactions may, therefore, be exploited in clinical analyses and point-of-care diagnostics for cancer, particularly if computational methods are used to process the data.
Highlights
IntroductionWith a molecular weight from 104 to 108 Da, depending on its origin [1]
Hyaluronic acid (HA) is a linear glycosaminoglycan comprising repeating disaccharide units of d-glucuronic acid (1-β-3) N-acetyl-d-glucosamine (1-β-4)with a molecular weight from 104 to 108 Da, depending on its origin [1]
We report on the detection of prostate cancer (PC3 line) cells using layer-by-layer (LbL) films [26] containing HA to take advantage of biospecific HA-CD44 interactions
Summary
With a molecular weight from 104 to 108 Da, depending on its origin [1]. It is found throughout the body but most concentrated in the vitreous of the eye [2], in the synovial fluid, and in the extracellular matrix of cartilages [3]. Cells 2020, 9, 1563 and tissues due to its rheological properties [5]. Cell processes involving HA include proliferation [7], locomotion [8], recognition, and differentiation [9]. The biocompatible properties of HA have been explored for clinical applications, especially in building blocks for the design of advanced materials
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
