Abstract
A small amount of water-soluble fructan (F) was formed from sucrose by 71 strains of Bacillus laevolacticus (I) and some strains of Sporolactobacillus inulinus. A gelatinous mass of water insoluble glucan (G), together with F, was formed from sucrose by a few strains of I. Polysaccharide formation was not observed in such groups of spore- bearing lactic acid bacteria as Bacillus coagulans, Bacillus racemilacticus and racemic lactic acid-producing Sporolactobacillus. Aeration was not needed for the formation of either F or G. Yield of G was up to 25% from sucrose and 2% from yeast extract-peptone broth with 8% sucrose. G was solubilized in 0.1N sulfuric acid by heating at 100° for 20min, and the solubilized G (SG) was partially hydrolyzed by dextranase. Molecular weights of SG and F were about 6.4×105 and more than 2×107, respectively. Antitumor activity of both SG and F against mouse Sarcoma 180 was about 60%.
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