Abstract
To investigate the protective effect of purified fraction 1 polysaccharide extracted from Rheum tanguticum RTP1 on irradiation-induced immune damage in mice. Kunming mice were randomly divided into five groups: normal group (NC), irradiation control group (IC), RTP1 low dose (200 mg/kg), middle dose (400 mg/kg) and high dose (800 mg/kg) groups. RTP1 was administered by the gastric route for 14 d, mice in the NC and IC groups being given by 0.9% sodium chloride solution in the same way. The mice in all groups except NC group were irradiated with 2.0 Gy⁶⁰Co γ-ray on the fourteenth day. Immune indives of non-specific immune function, cellular immunity and humoral immunity were assessed at the 24th hour after radiation. Compared with the IC group, the spleen index, thymus index, rate of carbon clearance, phagocytic function of macrophages, lymphocyte proliferation, hemolysin value of blood serum and NK activity were increased markedly (P < 0.05 or P < 0.05). RTP1 has an obvious protective effects on damage in γ-ray radiated mice.
Highlights
Radiation therapy is one of the most important strategies in cancer treatment with irreplaceable advantages
The spleen lymphocyte proliferation in irradiation control group (IC) group was significantly lower than the NC group (P < 0.05)
Compared with the IC group, the spleen cell proliferation in RTP1 groups was significantly higher than the IC group (P < 0.05)
Summary
Radiation therapy is one of the most important strategies in cancer treatment with irreplaceable advantages. The immune system damage could further weaken the immune function and leave the tumor out of control. The research on radioprotector has been the hot spot in radiation therapy. All of them have been verified that they could enhance immunity. We isolated and extracted five kind of RTP (Rheum tanguticum polysaccharide, RTP) from Rheum tanguticum with different molecular weight, and we verified that component 1 (RTP1, molecular weight 6×105 ~ 8×105) could promote the proliferation of mouse splenocytes, and coordinate to enhance the concanavalin (ConA)-induced T cell proliferation and bacterial lipopolysaccharide (LPS)-induced B cell proliferation (Jia et al, 2002; Zhang et al, 2006; Liu et al, 2009). The studies will provide the basis for the discovery of new anti-radiation drugs
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
