Polysaccharide Extracted from Bletilla striata Promotes Proliferation and Migration of Human Tenocytes.

Zhi-Yu Chen,Shih-Heng Chen,Feng-Huei Lin,Pang-Yun Chou,Chun-Chen Yang,Chih-Hao Chen

doi:10.3390/polym12112567

Abstract

Tendon healing after injury is relatively slow, mainly because of the weak activity and metabolic properties of tendon cells (tenocytes). Bletilla striata polysaccharide (BSP) has been reported to enhance cell proliferation. Here, we aimed to increase tendon cell proliferation by BSP treatment. We isolated tenocytes from the flexor tendon of human origin. Moreover, we improved the process of extracting BSP. When human tenocytes (HTs) were treated with 100 μg/mL BSP, the MEK/ERK1/2 and PI3K/Akt signaling pathways were activated, thereby enhancing the proliferation ability of tenocytes. BSP treatment also increased the migration of HTs and their ability to secrete the extracellular matrix (Col-I and Col-III). In conclusion, BSP was successfully extracted from a natural Chinese herbal extract and was shown to enhance tenocytes proliferation, migration and collagen release ability. This study is the first to demonstrate improved healing of tendons using BSP.

Highlights

The tendon is a soft and dense tissue that mechanically links muscle and bone
Results are shown in Figure 6E; PD98059 inhibited Bletilla striata polysaccharide (BSP)-induced proliferation of human tenocytes (HTs) (p < 0.05) and was greater after 24 h—27.6 and 22.4 times greater than extracellular signal-regulated kinase1/2 (ERK1/2) and Akt by 47%
Results are shown in Figure 6E; PD98059 inhibited BSP-induced proliferation of HTs by 47% compared with BSP group and wortmannin inhibited it by 60%, while co-treatment of both inhibitors inhibited

Summary

Introduction

The tendon is a soft and dense tissue that mechanically links muscle and bone. It is made up of tenocytes (or tenoblasts) and extracellular matrix (ECM), which predominantly comprises type I collagen. Tendon injuries are quite common and were reported in 54.8% of patients with a small laceration and 92.5% of patients with a deep injury in 2005 [6]. More than 30 million musculoskeletal injuries occur worldwide, of which over half are tendon and ligament injuries, and the actual number of injuries is likely to be even higher because many are not recorded [7,8,9]

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