Abstract
BackgroundDendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents. Polysaccharides are the main medicinal ingredients, yet no studies have been published on polysaccharide biosynthesis in D. moniliforme. To comprehensively investigate the polysaccharide at the transcription level, we performed de novo transcriptome sequencing for the first time to produce a comprehensive transcriptome of D. moniliforme.ResultsIn our study, a database of 562,580 unigenes (average length = 1115.67 bases) was generated by performing transcriptome sequencing. Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs). In the cellulose synthase family, 21 differential expression genes (DEGs) related to polysaccharide were identified. Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level. The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.ConclusionsWe determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing. This database provides a pool of candidate genes involved in biosynthesis of polysaccharides in D. moniliforme. Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.
Highlights
Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents
The stem of D. moniliforme is its main medicinal part and polysaccharide was mainly concentrated in stems
From one-way analysis of variance, it is shown that the polysaccharide content of the stem of D. moniliforme was significantly different from that of roots and leaves
Summary
Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents. Dendrobium moniliforme (Linnaeus) Swartz, is one of the most widespread species in the Orchidaceae family, which is a widely cultivated medicinal crop around Asia [1]. Yuan et al BMC Plant Biology (2019) 19:521 and are commonly used in the research and development in medicine and health food. They have pharmacological effects of regulating immunity, and acting against tumors and oxidants. Polysaccharides are natural macromolecules consisting of multiple monosaccharides units They represent a structurally diverse class of macromolecules that are widely distributed in nature and play an important role in controlling cell division, regulating cell growth and maintaining normal metabolism of living organisms. Mannose and glucose are the main monosaccharides in these Dendrobium species
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