Abstract
BackgroundLentinula edodes, known as shiitake, has been utilized as food, as well as, in popular medicine, moreover, compounds isolated from its mycelium and fruiting body have shown several therapeutic properties. The aim of this study was to determine the antiviral activity of aqueous (AqE) and ethanol (EtOHE) extracts and polysaccharide (LeP) from Lentinula edodes in the replication of poliovirus type 1 (PV-1) and bovine herpes virus type 1 (BoHV-1).MethodsThe time-of-addition assay was performed at the times -2, -1, 0, 1 and 2 h of the infection. The virucidal activity and the inhibition of viral adsorption were also evaluated. Plaque assay was used to monitor antiviral activity throughout.ResultsThe AqE and LeP were more effective when added at 0 h of infection, however, EtOHE was more effective at the times 1 h and 2 h of the infection. AqE, EtOHE and LeP showed low virucidal activity, and the inhibition of viral adsorption was not significant.ConclusionsThe results allowed us to conclude that AqE, EtOHE and LeP act on the initial processes of the replication of both strains of virus.
Highlights
Lentinula edodes, known as shiitake, has been utilized as food, as well as, in popular medicine, compounds isolated from its mycelium and fruiting body have shown several therapeutic properties
The polysaccharide isolated from Lentinula edodes contains high levels of b-D-glucan
The results demonstrated that both extracts, as well as, LeP, inhibited the replication of poliovirus type 1 (PV-1) and bovine herpes virus type 1 (BoHV-1)
Summary
Known as shiitake, has been utilized as food, as well as, in popular medicine, compounds isolated from its mycelium and fruiting body have shown several therapeutic properties. There are a little more than 40 drugs approved for clinical use in the treatment of viral infections [1] Studies regarding these drugs concentrated on synthetic products up to the end of the 80 s, when natural compounds attracted attention because of their efficacy in the inhibition of viruses important for human and animal health [2]. Poliovirus is a non-enveloped virus with an icosahedral capsid symmetry, and a genome consisting of a positive single-stranded RNA. Viron belongs to the subfamily Alphaherpesvirinae, family Herpesviridae [17], has a genome consisting of a linear double-stranded DNA within an icosahedral capsid, enclosed by an envelope. In order to reduce losses caused by BoHV, vaccines consisting of attenuated virus are being utilized with positive results. The control of infections is still difficult, due to the latency established by the virus after primary infection or after vaccination [18]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
