Abstract
EARLY germination of seed embryos is characterised by a rapid increase in polyribosome content and rate of protein synthesis1,2, and there is evidence3–8 that these events are a consequence of the mobilisation of preformed messenger RNA9. We observed ithat new mRNA synthesis could be measured at this time of rapid polyribosome formation10. Using cordycepin and α-amanitin, inhibitors of this mRNA synthesis, we have now confirmed ithat preformed mRNA has an important role in early germination, and have found that polyribosome formation at this time is independent of both transcription and of polyadenylation.
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