Abstract
In this research, the successful application of polypyrrole (PPy)-modified magnetic nanoparticles (NPs) is described as an efficient adsorbent for the extraction and the preconcentration of glibenclamide (GB). To measure it in biological fluids samples, HPLC-UV detection was used. First, iron oxide NPs were prepared by coprecipitation procedure and then their surface was modified by PPy monomers. Characteristics of Fe3O4@PPy NPs were investigated by FTIR technique and NP size studied with scanning electron microscopy. The vibrating sample magnetometer was used to characterise the magnetic properties of the prepared modified NPs. The affecting parameters in extraction including analyte sorption time, analyte desorption time, ionic strength, sample volume, pH, eluent type, eluent amount, and amount of Fe3O4@PPy NPs were investigated and optimised. The linear range of the proposed method is 0.2–700.0 μg l−1 and the limit of detection is 0.1 μg l − 1. The relative standard deviation for five replicate analyses was 3.9. Finally, the proposed procedure was successfully employed for preconcentration and determination of GB in biological fluids.
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