Abstract
Polyploid induction has been conducted by different colchicines concentrations with seed, hypocotyl and apices of Lespedeza formosa. Morphological variation and amounts of chromosome about the polyploidy have also been analyzed. Results show that the best material for polyploid induction of L. formosa is the new apices from seed germination, with an induction rate of 44.4% at 0.1% colchicines concentration and 36 hours of treatment time. Compared with normal diploid plants, the polyploidy plants of L. formosa inducted in our experiments have short stature and stems with thick and wide leaves. Cytological studies show that the changed seedlings, whose morphology has changed dramatically, are tetraploid, with 44 (2n = 4x = 44) chromosomes in the somatic cells.
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