Abstract
Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging.
Highlights
There is evidence that oxidative stress, exerting downstream effects such as lipid peroxidation, DNA damage and mitochondrial impairment, may have a causative role in skin disease and aging [1,2].The skin is the largest organ of the body that creates a self-repairing barrier, protecting the body from the most common potentially harmful physical, environmental, and biological insults
According to our previous results [1], the Sveva cultivar is a remarkable source of phenols and flavonoids (Table 1); five anthocyanin pigments were detected through HPLC-DAD/ESI-mass spectrometer (MS) analysis, with Pg- and Cy-glycosides being the most representative anthocyanin strawberry components (Table 1)
Our results report that the Sveva strawberry cultivar has a high antioxidant capacity, as well as an important anthocyanin and vitamin content, which results in a protective effect on skin cells against damage induced by oxidative stress
Summary
There is evidence that oxidative stress, exerting downstream effects such as lipid peroxidation, DNA damage and mitochondrial impairment, may have a causative role in skin disease and aging [1,2].The skin is the largest organ of the body that creates a self-repairing barrier, protecting the body from the most common potentially harmful physical, environmental, and biological insults. In recent years several studies have shown that oxidative stress and senescence in human fibroblasts can be counteracted and delayed by certain protective antioxidant compounds [1,4,7]: dietary vitamins and polyphenols are able to ameliorate oxidative skin damage and prevent aging, even if the role of diet in protecting the skin is highly controversial due to the limited amount of scientific data available [8]. In the last few years topical treatment of these compounds has been proposed as a strategic tool in order to provide additional protection from oxidative damage and delay skin aging [8,9]
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