Abstract
An ultrasonic-assisted extraction (UAE) method was adopted to extract natural antioxidants from edible Gordonia axillaris fruit. Single-factor experiments and response surface methodology were conducted to investigate the influences of five different parameters on antioxidant capacity. The optimal conditions of the UAE were 39.78% ethanol, 30.94 mL/g solvent/material ratio, 59.47 min extraction time, 40 °C temperature, and 400 W ultrasonication power. The antioxidant capacity was 525.05 ± 14.34 µmol Trolox/g DW under the optimal conditions, which was in agreement with the predicted one (531.71 µmol Trolox/g DW). Additionally, in comparison with two traditional methods (maceration and Soxhlet extraction), the established UAE method greatly improved the yield of antioxidants and significantly reduced the extraction time. Besides, nine phenolic compounds were identified and quantified in the extract of Gordonia axillaris fruits by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), including rutin, gallic acid, protocatechuic acid, epicatechin, 2-hydrocinnamic acid, p-coumaric acid, quercetin, epicatechin gallate, and ferulic acid. The richness of phenolic compounds in the Gordonia axillaris fruits indicated its potential health benefits, and its extract rich in antioxidants could be developed into functional food or nutraceuticals with the potential to prevent certain diseases induced by oxidative stress, such as cardiovascular diseases and cancers. This study also provided a way to enhance the economic values of Gordonia axillaris fruits compared to raw fruits.
Highlights
Many fruits have been found rich in phenolic compounds, which have been reported to possess antioxidant, antimicrobial, and anti-inflammatory activities [1,2]
Compared to the raw fruits, Gordonia performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) was axillaris extracts can be concentrated in natural antioxidants, which Compared can be usedtoas adoptedfruit to identify and quantify main phenolic compounds in the extract
Thehigh-value raw fruits, Gordonia axillaris fruit extracts can be concentrated in natural antioxidants, which can be used as highfunctional ingredients or additives to formulate different functional foods or nutraceuticals with the potential to prevent certain diseases related to oxidative stress
Summary
Many fruits have been found rich in phenolic compounds, which have been reported to possess antioxidant, antimicrobial, and anti-inflammatory activities [1,2]. Gordonia axillaris fruit is critical for its value-added the fruits, developing an efficient method to extract antioxidants, especially the phenolic compounds, utilization. The present study aimed at establishing efficient effective concentration bioactive compounds couldisbe reached more easilyanby intake UAE of themethod extract to extract natural antioxidants. Compared to the raw fruits, Gordonia performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) was axillaris extracts can be concentrated in natural antioxidants, which Compared can be usedtoas adoptedfruit to identify and quantify main phenolic compounds in the extract. Thehigh-value raw fruits, Gordonia axillaris fruit extracts can be concentrated in natural antioxidants, which can be used as highfunctional ingredients or additives to formulate different functional foods or nutraceuticals with the potential to prevent certain diseases related to oxidative stress
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
