Abstract
Polyphenolic compounds in the lupulin glands of the female inflorescence of Humulus lupulus were identified. It was demonstrated that only those possessing 3′,4′-dihydroxyphenol groups were capable of inducing a lag phase in the enzymic oxidation of alpha-acids during which the phenolic compound was itself altered by the enzyme. Examination of the kinetics of interaction between the resin substrate, phenolic inhibitor-substrate and the enzyme provided a basis for the suggestion that the system may represent a tolerance mechanism (towards a product of secondary metabolism which is stored in an extracytoplasmic compartment) in secretory cells.
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