Polyphenolic extract from Artemisia selengensis Turcz ameliorates experimental oral mucositis

Fahu Yuan,Li Liu,De Chen,Wenxuan Yu,Beibei Zhu,Siyuan Sun,Yanbing Jiang,Changli Zeng,N Hamid,S Zhou

doi:10.1051/e3sconf/202018902019

Abstract

Oral mucositis is a common and frequentoccurring disease and there is no effective treatment. In this study, we investigated the efficacy of polyphenolic extract from Artemisia selengensis Turcz (PPAST) in the prevention and treatment of oral mucositis. Cultured human gingival fibroblast cell HGF-1 was used as an in vitro experimental model to confirm the effect of PPAST inhibition of lipopolysaccharide(LPS) on cytotoxicity and its effect on the production of inflammatory cytokines. A rat model of oral ulcer was induced by acetic acid cauterization, and the curative effect of PPAST on oral ulcer was investigated from ulcer area and ulcer duration. PPAST significantly inhibited the toxic effect of LPS on HGF1 cells, improved the survival rate of HGF1 cells, and showed a concentration-dependent inhibition of TNF-α in HGF-1 cell. Treatment with PPAST reduced mucositis scores, promoted oral ulcer healing, and reduced plasma TNF-α levels in rats. Experimental data show that PPAST is safe and effective in the prevention and treatment of oral inflammatory diseases.

Highlights

Oral inflammatory diseases are common and frequentlyoccurring diseases
The leaves of Artemisia selengensis Turcz were dried at 60 °C and crushed, and every 20 gram of Artemisia selengensis Turcz leaves were added with 300 mL 75% ethanol extract solution, mixed, treated by ultrasonic wave for 35 minutes, stood for 20 minutes, centrifuged at 5000 RPM for 15 minutes, the supernatant was taken, concentrated by rotary evaporation and freeze-dried, and the PPAST obtained was stored in the dryer for use
3.1 PPAST alleviates the effects of LPS-induced human gingival fibroblast toxicity

Summary

Introduction

Oral inflammatory diseases are common and frequentlyoccurring diseases. Almost everyone has the experience of suffering from oral inflammatory diseases, such as Oral mucositis, oral ulcers, periodontitis, gingivitis and periodontitis. Oral inflammatory diseases are self-healing to a certain extent, oral inflammation can cause discomfort, swelling, pain or bleeding, and seriously affect work and life. Periodontal inflammation refers to the inflammation of periodontal tissues such as gum, periodontal membrane and alveolar bone. In the pathological process of periodontal inflammation, LPS has obvious cytotoxic effect on gingival fibroblasts, the main cell of human periodontal tissue, inhibiting the growth of gingival fibroblasts, cell proliferation and DNA synthesis. LPS stimulates macrophages, monocytes, fibroblasts and periodontal tissue cells to produce and secrete various inflammatory mediators, such as PGE 2, IL-1β, TNF-α and IL-6. Under the action of inflammatory mediators, it causes enhanced vascular permeability and leukocyte infiltration, inhibits collagen synthesis, and causes gingival bleeding and gingival swelling and pain[3]

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