Polyphenol-rich extract of Vernonia amygdalina (Del.) leaves ameliorated cadmium-induced alterations in feeding pattern and urine volume of male Wistar rats.

Abstract

Aim:To determine the effects of a polyphenol-rich extract of the leaves of Vernonia amygdalina (PEVA) on the feeding pattern of rats that are exposed to cadmium (Cd) toxicity.Materials and Methods:Thirty male Wistar rats, weighing 160-180 g, were divided into 6 groups of 5 rats each as follows; Group 1 received distilled water orally (0.2 ml a 100 g rats), daily, throughout the period of study. Group 2 received Cd alone (in the form of CdSO4) at 5 mg/kg/day via intraperitoneal route for 5 consecutive days. Group 3 were pre-treated with Cd as Group 2 and thereafter left untreated for a period of 4-week. After the oral lethal dose of PEVA was determined, Groups 4, 5, and 6 received graded doses of PEVA at 100, 200 and 400 mg/kg/day (0.2 ml per 100 g rats), respectively via oral route for 4 weeks after they were pre-treated with Cd as Group 2. Blood samples were collected for some plasma biochemical assays while urine samples were collected using metabolic cages.Results:PEVA administration significantly increased (P < 0.05) the body weight and feeding patterns that were significantly reduced (P < 0.05) by Cd toxicity. PEVA also significantly reinstated the plasma antioxidant status, as well as glucose and urine volume of the rats toward control values (P < 0.05).Conclusion:PEVA can be an herbal alternative in the treatment or management of subjects manifesting alterations in feeding pattern and urine volume that is Cd-induced.