Polyphenol loaded nanoparticles as antiglycating agents: A case study with human serum albumin

Abstract

Advanced glycation end products (AGEs) formed through nonenzymatic glycation results in diseases including diabetic complications, Alzheimer’s disease and atherosclerosis etc. The development of novel antiglycating agents has thus become an important area of research. In our present work, we have reported the inhibitory activity of morin loaded PLGA NPs (MPNPs) on the D-ribose induced glycation of human serum albumin (HSA) based on spectroscopic studies. MPNPs were characterized by spectroscopic and microscopic techniques. HSA was incubated with ribose at 37 °C for one month and the formation of AGEs was confirmed from fluorescence studies, the increase in absorbance and the measurement of free amino groups. Further HSA and ribose mixtures incubated with MPNPs showed significant reduction in the extent of advanced glycation end product formation that were confirmed by UV-Vis and fluorescence spectroscopy and matrix-assisted laser desorption/ionization time-of-flight (MALDI- ToF) techniques. The structural changes induced by MPNPs were further confirmed by circular dichroism studies. Our findings suggest that MPNPs have the potential to be used antiglycating agents.