Polyphenol Extract from Evening Primrose (Oenothera paradoxa) Inhibits Invasion Properties of Human Malignant Pleural Mesothelioma Cells.

Malgorzata Chmielewska-Kassassir,Wojciech M Ciszewski,Wieslaw Wiczkowski,Malgorzata Bukowiecka-Matusiak,Dorota Szczesna,Lucyna A Wozniak,Waldemar Wagner,Izabela Burzynska-Pedziwiatr,Katarzyna Sobierajska

doi:10.3390/biom10111574

Malgorzata Chmielewska-Kassassir, Wojciech M Ciszewski + Show 7 more

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https://doi.org/10.3390/biom10111574

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Abstract

Extracts from the defatted evening primrose (Oenothera paradoxa Hudziok) seeds are the source of a range of stable polyphenolic compounds, including ellagic acid, gallic acid, and catechin. Our studies evaluate, for the first time, the influence of evening primrose isopropanol extract (EPE) on malignant pleural mesothelioma (MPM) cells. MPM is rarely diagnosed, its high aggressiveness and frequently noted chemoresistance limit its treatment schemes and it is characterized by low prognostic features. Here, we demonstrate that EPE inhibited MPM growth in a dose-dependent manner in cells with increased invasion properties. Moreover, EPE treatment resulted in cell cycle arrest in the G2/M phase and increased apoptosis in invasive MPM cell lines. Additionally, EPE strongly limited invasion and MMP-7 secretion in MPM cancer cells. Our original data provide evidence about the potential anti-invasive effects of EPE in MPM therapy treatment.

Highlights

Phytotherapy has been used in human cultures from ancient ages, a renewed interest has been observed in the last two to three decades
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The chemical composition of EPE reflects the presence of compounds belonging to different polyphenols classes including catechin and procyanidins, penta-O-galloyl-β-d-glucose as well as di- and tetragalloyl glucose belonging to hydrolyzable tannins, different phenolic acids, and flavonols

Summary

Introduction

Phytotherapy has been used in human cultures from ancient ages, a renewed interest has been observed in the last two to three decades. The extracts obtained from defatted seeds of Oenothera sp., including O. paradoxa, contain biologically active chemicals, such as gallic acid (GA), ellagic acid (EA), or penta-O-galloyl-β-d-glucose (PGG). These compounds reveal potential antitumor effects manifested by the inhibition of cancer development [4,5,6]. Gallic acid is a natural plant triphenol Both GA and its derivatives show antitumor activity against a variety of human cancer cells, including lung, breast, prostate, and skin lineages [7,8,9,10]. Supplementing standard anticancer agents with natural extracts rich in polyphenolic compounds, including evening primrose extract (EPE), seems to be one of the promising solutions managing tumor cells

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