Abstract

AbstractThe aim of the study was to compare the content of selected phytochemicals as well as the antioxidant and antimicrobial potential of the leaves of Prunus padus L. and Prunus serotina L., as there is very little research on this subject in the literature. Therefore, it is used to deepen knowledge on this subject. In addition, an electrochemical test was also carried out, which was not yet available for the above plants. Antibacterial studies have also been deepened to include the analysis of new strains of bacteria and fungi, which has not been studied earlier. The water extracts of P. padus using the utra-performance liquid chromatography (UPLC) system showed a higher content of both phenolic acids and flavonols (651.77b ± 18.12 mg/100 g dw for acids and 3.85b ± 0.08 mg/100 g dw for flavonols, respectively). Ferulic and p-coumaric acids were the dominant polyphenols in leaves. Extracts from P. padus showed higher activity against DPPH radical, which was 6.62b ± 0.06 mg TE/1 g dw, as well as higher antioxidant capacity, measured using 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) cation radical (37.39b ± 3.81 mg TE/g dw). The higher antioxidant potential of P. padus was confirmed based on the oxidizing potentials of electroactive compounds present in them. Stronger inhibition against Enterococcus faecium and Klebsiella pneumoniae was found for P. padus, whereas P. serotina extract was more potent against Enterococcus faecium bacterium. It has been shown that P. padus can be an attractive raw material with antioxidant and antimicrobial properties that can be used on a much wider scale in food technology than its current application.

Highlights

  • Natural products of plant origin are gaining interest worldwide due to component contents that may affect health

  • 2.8 Antimicrobial activity testing using the well-diffusion method. Indicator microorganisms such as Gram-negative bacteria: Klebsiella pneumoniae (ATCC 31488), Salmonella enteritidis (ATCC 860), Pseudomonas aeruginosa (ATCC 27853), and Acinetobacter baumannii (ATCC 19606) and Gram-positive bacteria: Enterococcus faecium (ATCC 27270), Enterococcus faecium (ATCC 27270), Staphylococcus aureus (ATCC 25923), Lactobacillus fermentum (ATCC 14932), Clostridium butyricum

  • Bird cherry leaves are a good source of polyphenolic compounds with high antioxidant activity

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Summary

Introduction

Natural products of plant origin are gaining interest worldwide due to component contents that may affect health. Bird cherry (P. padus) and black cherry (P. serotina) are popular plants found in many regions in the world. They have fruits with a bitter aftertaste, which are most often used as raw material for making tinctures. Bird cherry was considered as a medicinal plant. It has been shown that the fruits of P. padus contain organic acids, minerals, and vitamins, mainly vitamin C. The most important flavonols found in the fruits of Prunus padus L. were quercitrin and quercetin, epicatechin and catechin dominated. Prunus is considered as an invasive species.

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