Abstract
During inflammation, reactive oxygen species (ROS) are produced in large amounts, causing oxidative stress. Several studies confirm that plant extracts rich in phenolic compounds may inhibit ROS production. For that reason, the aim of this work is the qualitative and quantitative analysis of phenolic acids and flavonoids in the etheric (LAEN) and methanolic (LAM) extracts of the fruits of Laserpitium krapffii Crantz, as well as their antioxidative and cytotoxic properties. Liquid chromatography–electrospray tandem mass spectroscopy (LC-ESI-MS/MS) enabled the identification of 12 phenolic acids and nine flavonoids. Both tested extracts scavenged ROS in a concentration-dependent manner. Stronger activity was observed for the methanolic extract. The cytotoxic effect of both extracts in increasing concentrations on five types of cancer cell lines was also investigated. The cytotoxicity was estimated using trypan blue vital staining. It was found that the analyzed extracts induced the apoptosis of the cells of all the tested cell lines. In conclusion, our results present that the fruits of L. krapffii can be a source of valuable compounds with protective effects against oxidative damage.
Highlights
The main group of secondary metabolites occurring in the Laserpitium L. genus is sesquiterpene lactones, which are mainly of the slovanolide-type class of guaianolides [3,5]
The amounts of phenolic compounds in the tested extracts were proportional to the intensity of absorption measured spectrophotometrically
The analysis indicated that the methanolic extract contains much larger quantities of total phenolic, flavonoids, and phenolic acids content
Summary
Kit.) is a perennial herbaceous plant that is commonly known as Krapf’s laserwort. Reports regarding the chemical composition and biological activities of L. krapffii are limited. Data indicated the content of only sesquiterpene lactones [2,3] and essential oils [4] in this plant. The main group of secondary metabolites occurring in the Laserpitium L. genus is sesquiterpene lactones, which are mainly of the slovanolide-type class of guaianolides [3,5]. The exception is L. krapffii; these compounds are isolated from the herb, fruits, and underground parts of L. siler [3,6], the rhizomes and roots of L. orchidanum and L. zerney [5], L. archangelica [7], and L. prutenicum [8]
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