Abstract
The polypeptide composition of incomplete WSN influenza virions grown in MDBK cells is altered relative to a control grown at low m.o.i. The relative amount of viral glycoproteins in the third and fourth serial undiluted passages were increased to approximately twice the level in control virus, while the NP and M proteins were decreased. Neuraminidase activity was also increased, suggesting that the level of NA (neuraminidase) glycoprotein is also higher in incomplete virions. The increase in neuraminidase activity was already evident in the first undiluted passage, where no substantial increase in overall glycoprotein content was demonstrated. Similar alterations in protein composition were seen in several different preparations exhibiting different morphological aberrations and in incomplete particles that were of normal morphology. These results permitted the following conclusions regarding incomplete WSN virus grown in MDBK cells: (1) Neuraminidase and HA (hemagglutinin) glycoprotein levels are regulated independently in the incomplete virion; (2) the level of these proteins in the virion can be influenced by growth conditions; (3) the glycoproteins do not play a primary role in determining particle morphology.
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