Abstract
In virus-infected or transformed cells, polyoma expresses a large number of functions. Attempts to understand these in relation to the structure of the virus DNA have until recently been limited by our knowledge of polyoma DNA anatomy and nucleotide sequence. Methods such as denaturation mapping, based on the variation of base composition in different regions of the DNA, provide some information, but they are limited by the small size and circularity of the molecule (Follet and Crawford 1968). Cleavage by restriction endonucleases, which cut the DNA at specific nucleotide sequences, provide both better reference points on the DNA and the means for physical separation of different regions. Here we have made use of a variety of restriction enzymes in conjunction with other techniques to provide information on the mode of DNA replication and on the positions in the molecule at which replication starts and finishes.
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