Polymyxin B-immobilized fibre haemoperfusion reduces urinary 8-hydroxy-2′-deoxyguanosine levels in patients with septic shock

Abstract

Abstract Objective. To determine whether urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels are altered in patients with septic shock and whether polymyxin B-immobilized fibre (PMX-F) haemoperfusion reduces the 8-OHdG level in these patients. Design and subjects. Twenty patients with septic shock and 20 age-matched healthy volunteers were included in the study. The septic shock patients were divided into two treatment groups: a PMX-F treatment group (n =12) and a non-PMX-F treatment group (n =8). Standard supportive care was continued without change during PMX-F haemoperfusion. Clinical markers, including plasma endotoxin, were measured before and after the first and the second PMX-F treatment and the following day. Urinary 8-OHdG levels were also examined before and after the first and the second PMX-F treatment (24 h pooled urine) and the following day. Results. Urinary 8-OHdG levels were significantly higher in septic shock patients (median 38.0 ng/mg creatinine; range 16.0–52.0 ng/mg creatinine) than...