Abstract
The optimal dialysis procedure for treatment of end-stage renal disease in children is still controversial. By using the PML model we have studied the different influences of regular hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) on cell metabolism. We determined protein synthesis (PS, by 3H-LEU incorporation), intracellular amino acids (AA, by reverse phase HPLC), glycolytic enzymes (PK, PFK) and energy rich adenine nucleotides in PML of 26 patients aged 2-22 yrs either on HD (n=8) or on CAPD (n=18) compared to controls (C, n=20). PS was more reduced on HD (m±SEM:699±220 pmoles/h/mg DNA, p < 0.02) than on CAPD (1184±173 vs 1649±217 in C, NS). Profound alterations were observed in intracellular AA in both CAPD and HD. Activities of PK (means: HD 12678*/CAPD 8457/C 6314 u/mg DNA) and PK (134*/179*/104 u/mg DNA) were significantly* increased in patients. Energy change derived from adenine nucleotide levels was significantly increased on CAPD (0.918 vs 0.896 in C). These results suggest improved PS by CAPD compared to HD.
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