Polymorphisms of exon 10 in the BPI gene and its association analysis with some reproductive traits and partial immune indexes in Meishan pigs

Huan Qu,Shenglong Wu,Lan Ye,Zongyou Wei

doi:10.3906/vet-1807-89

Abstract

This study was conducted to detect polymorphisms of BPI gene exon 10 by PCR-RFLP in Meishan pigs and their associations with some important cytokine levels (IL-1s, IL-4, IL-6, IL-8, IL-10, TGF-1s, IFN-γ, TNF-α) and reproductive traits (total number born (TNB), number born alive (NBA), birth litter weight (BLW), and weaning litter weight (WLW)), with the aim of identifying effective genetic markers for molecular breeding. The results revealed three genotypes, including AA, AB, and BB, with the trend of AA > BB > AB in reproductive traits. Pigs with the AA genotype were significantly higher than the AB genotype in TNB and IFN-γ (P 0.05). Comprehensive analysis indicated that the polymorphisms of exon 10 in the BPI gene have significant genetic effects on some reproductive traits and cytokines in Meishan pigs, for which the AA genotype was demonstrated to be the favorable genotype, which could be used as potential genetic markers for in-depth research and examination.

Highlights

Bactericidal/permeability-increasing protein (BPI) is an endogenous antibacterial protein, which is mainly found in human and mammalian aniline blue particles of polymorphonuclear leukocytes [1]
Genotype distribution and allele frequency of BPI gene in Meishan pigs The PCR-RFLP method was used to detect the genotypes of Meishan pigs
Recent studies have reported the associations of the porcine BPI gene and disease resistance

Summary

Introduction

Bactericidal/permeability-increasing protein (BPI) is an endogenous antibacterial protein, which is mainly found in human and mammalian aniline blue particles of polymorphonuclear leukocytes [1]. BPI is a member of the lipopolysaccharide binding protein family and an antimicrobial active protein. It plays an important role in the natural defense systems of animals [2]. Studies have demonstrated that the BPI protein has multiple biological functions including promotion of complement activation, inhibition of inflammatory mediators’ release, and infection by fungal and parasitic organisms. It resists and kills bacteria (especially gram-negative bacteria) and neutralizes endotoxins or lipopolysaccharide (LPS) [3,4,5]. Given the regulatory role of the BPI gene in killing gram-

Methods

Results

Conclusion