Polymorphisms of adiponectin rs150299 and rs266722 and insulin sensitivity in pregnant Mexican women

Abstract

Lower adiponectin concentrations are associated with impaired glucose tolerance (IGT) and type 2 diabetes(T2DM). Hispanics have increased genetic risk for IGT & T2DM. Adiponectin SNPs rs266729 and rs 1501299 are correlated with adiponectin concentrations in Hispanics. We measured adiponectin SNPs & fasting adiponectin (FA), insulin (FI)& glucose (FG) in 91 non‐diabetic pregnant Mexican women at 20 ±2 & 28±2 wks gestation. RT‐PCR was performed after DNA extraction. HOMA‐IR was calculated. HOMA‐IR, FI & FA concentrations differed significantly between 20 & 28 wks (FI 12.7 vs 15.4 uU/mL, p<0.0001; HOMA‐IR 2.5 vs 3.0, p<0.0001; FA 12.5 vs 11.7, p<0.0007). After controlling for age, pre‐pregnancy BMI and parity, the changes between 20‐28 wks in FI (F=5.57, p<0.0053), FG (F=3.54, p<0.0335) and HOMA‐IR (F=5.92, p<0.0039) differed between rs266729 CC vs CG SNPs. No significant differences in FA were observed in the rs1501299 or rs266729 genotypes. The effect of SNP rs266729 on changes in HOMA‐IR, FI and FG, but not on FA, suggests that this SNP may affect insulin sensitivity independently of changes in total FA. Alternatively, one or more multimeric forms of adiponectin may be involved in insulin sensitivity in pregnancy independently of total FA. Funded by Bristol Myers Squibb