Polymorphisms of 5’-UTR of rad51 gene in prostate cancer

Mazhar Salim Al-Zoubi,Ismail Matalka,Sohaib M Al-Khatib,Mohammed Alorjani,Riyad Muhaidat,Mutaz Abd Al-Razaq,Khalid Al-Batayneh,Mohammad Al Hamad,Raed Al-Zoubi,Bahaa Al Trad,Samir Al Bashir

doi:10.17816/ecogen16224-29

Abstract

Background. Notwithstanding that prostate cancer is largely studied all over the world for many decades, its etiology is not known and there is an intensive work to elucidate the cause and molecular markers for the development of this male cancer. Polymorphisms in DNA repairing genes may affect the DNA repairing capacity that in turn contributes to cancer development. This study aims to explore the polymorphisms of homologous recombination (HR) RAD51 gene (rs1801320 and rs1801321) as a possible risk factor for developing prostate cancer. Sequencing of 5'-UTR of RAD51 gene (rs1801320 and rs1801321) was studied in 80 DNA samples of prostate cancer and 50 DNA samples from a control group. Our results revealed a significant correlation between rs1801320 GC polymorphism and the presence of prostate cancer in the Jordanian population (p = 0.041, X2 = 6.377). On the other hand, the rs1801321 GT polymorphism was not associated with the presence of prostate cancer in the study population (p = 0.27, X2 = 2.6). In conclusion, our results shed a light on the possible role of RAD51 gene polymorphisms in the development of prostate cancer; however, a larger representative study is needed to elucidate a possible role of RAD51 gene polymorphisms in development and prognosis of prostate cancer.

Highlights

Prostate cancer (PC) in Jordan, as the developed countries, is the most commonly diagnosed cancer in men
This study aims to explore the polymorphisms of homologous recombination (HR) RAD51 gene as a possible risk factor for developing prostate cancer
Our results showed a significant association between G135C variant and the development of prostate cancer in the Jordanian population

Summary

Introduction

Prostate cancer (PC) in Jordan, as the developed countries, is the most commonly diagnosed cancer in men. The incidence of prostate cancer in Jordan has increased in the last decades, and it shows a rise in the number of prostate cancer cases among Jordanian males from 123 in 2000 to 218 in 2010 [1]. As a heterogeneous type of cancer, prostate cancer has no identified susceptibility genes or etiologic agents [2,3,4]. Some genetic loci have been studied in prostatic carcinoma including BRCA1, BRCA2, ELAC2, RNASEL and MSR1 genes [3]. Low penetrance of those genes was demonstrated in prostate cancer [5,6,7]. Many studies have investigated the possible association between the mutations or polymorphisms in DNA repair genes and the development of prostatic carcinoma [8]

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