POLYMORPHISMS IN THE 5Â´ FLANKING REGION AND FIRST EXON OF INSULIN-LIKE GROWTH FACTOR 1 GENE IN SOUTHERN POPULATIONS OF BUFFALO

Khederzadeh Khederzadeh

doi:10.3844/ajbbsp.2013.348.354

Abstract

The Insulin-like Growth Factor 1 (IGF-1) system plays a critical endocrine role controlling nutrient metabolism in animals and it is a group of structurally-related polypeptides that regulate the growth of many types of mammalian cells. In liver, IGF-1 is dynamically regulated by lactation and energy balance. Less is known about the regulation of IGF-1 and IGF-binding protein mRNA in reproductive tissues and plays an important role in bovine follicular growth, acquisition of oocyte competence and embryo viability. The present study describes Polymorphisms in the IGF-1 Gene of Buffalo population in south of Iran. In order to characterization polymorphism, blood sample were collected from 95 buffalos from Ahvaz, Dezful, Shushtar, Shadegan and Susangerd cities. DNA extraction was based on Boom method and exon 1 of the IGF-1 gene was amplified to produce a 250 bp fragment. The amplified fragment were digested with Eco105I (SnaBI) restriction endonuclease. Also, the 265 bp of IGF-1 promoter in the 5’ Flanking Region was amplified and subsequently subjected to Single Strand Conformation Polymorphism (SSCP). The results were revealed one pattern (B) in digestion and all the five populations were monomorph. But, the populations exhibited three different SSCP patterns. It is possible that this gene has not exposed to selection and inbreeding is estimated high.

Highlights

Genetic evaluation of animal performance has been accomplished by quantitative approaches which depend on molecular technology for identifying genes of economically interesting traits and analysis of the polymorphism of genes whose products are key enzymes in the metabolic pathways of important physiological processes and are related to phenotypes (Beuzen et al, 2000)
The present study describes Polymorphisms in the Insulin-like Growth Factor 1 (IGF-1) Gene of Buffalo population in south of Iran
The amplified of promoter in the 5 ́ Flanking Region and exon 1 of IGF-1 Gene resulted in a DNA fragment with 250bp and 265bp with PCR technique (Fig. 2)

Summary

Introduction

Genetic evaluation of animal performance has been accomplished by quantitative approaches which depend on molecular technology for identifying genes of economically interesting traits and analysis of the polymorphism of genes whose products are key enzymes in the metabolic pathways of important physiological processes and are related to phenotypes (Beuzen et al, 2000). Candidate genes have known biological functions related to the development or physiology of an important trait (Rothschild et al, 1997). Such genes can encode structural proteins or a member in a regulatory or biochemical pathway affecting the expression of the trait (Bryne and McMullen, 1996) and can be tested as putative QTLs (Yao et al, 1996). Insulin-like growth factors 1 and 2 (somatomedins-IGF-1 and IGF-2) are structurally related proteins, playing a key role in cell differentiation, embryogenesis, growth and Corresponding Author: Saber Khederzadeh, Department of Genetic and Biotechnology, Faculty of Biological Sciences, Varamin-Pishva branch, Islamic Azad University, Varamin, P.O. Box 33817-74895, Iran

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