Polymorphisms in hormone-sensitive lipase and leptin receptor genes and their association with growth traits in Barki lambs.

Adel H. M. Ibrahim

doi:10.14202/vetworld.2021.515-522

Abstract

Background and Aim:Marker-assisted selection has many advantages over conventional selection in animal breeding. The candidate gene approach has been applied to identify genetic markers associated with economically important traits in livestock. This study was established to investigate variation in the hormone-sensitive lipase (HSL) and leptin receptor (LEPR) genes, and their association with growth traits in Barki lambs.Materials and Methods:Records for birth weight (BW), pre-weaning average daily gain (ADG1), weaning weight (WW), post-weaning average daily gain (ADG2), and marketing weight (MW) were obtained from 247 Barki lambs. Polymerase chain reaction–single-stranded conformational polymorphism analyses were used to detect variation in exon 9 of HSL and exon 19 of LEPR. General linear models were used to test for associations between the variation in ovine HSL and LEPR, and growth traits.Results:The SSCP banding patterns for HSL showed three variants (H1, H2, and H3), which contained two nucleotide-sequence differences (c.1865C>T and c.2038T>C). Two SSCP banding patterns (L1 and L2) were observed for LEPR and these contained two nucleotide-sequence differences (c.2800G>A and c.2978C>G). The HSL genotype showed no effect on the studied traits. The LEPR genotype was proven to have significant effects (p<0.05) on ADG2 and MW. The presence of the L1 variant was associated (p<0.01) with decreased ADG2 and MW.Conclusion:The finding of an association between LEPR gene variation and growth rate after weaning in Barki lambs warrants efforts to improve this trait.

Highlights

In Egypt, there are major problems associated with supplying sufficient food to the population, especially meat products
A total of two single-nucleotide polymorphisms (SNPs) (c.1865C>T and c.2038T>C) that were previously reported in the NBCI were detected by performing DNA sequencing of the three detected variants in exon 9 of the hormone-sensitive lipase (HSL) gene
As shown in Table-2 and Figure-3, two different SSCP banding patterns were observed for amplicons from the amplified region of leptin receptor (LEPR) in the Barki lambs and three combinations of SSCP patterns corresponding to three different genotypes were detected

Summary

Introduction

In Egypt, there are major problems associated with supplying sufficient food to the population, especially meat products. This has directed attention toward improving the productivity of livestock adapted to the conditions of arid and semi-arid areas, which constitute about 94% of Egypt’s land. In these areas, Barki sheep have advantages over large ruminants, in that they can utilize a wider diversity of plants and have a higher reproductive rate, allowing populations to recover more quickly than large ruminants. The candidate gene approach has been applied to identify genetic markers associated with economically important traits in livestock. This study was established to investigate variation in the hormone-sensitive lipase (HSL) and leptin receptor (LEPR) genes, and their association with growth traits in Barki lambs

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