POLYMORPHISM ROLE OF GNB3 GENE IN THE DEVELOPMENT OF LEFT VENTRICULAR HYPERTROPHY OF PATIENTS WITH PRIMARY ARTERIAL HYPERTENSION

Abstract

Abstract Objective: To analyze the distribution of polymorphic variants of the guanine gene of the binding G protein of the beta-3 subunit (GNB3) of patients with primary arterial hypertension; Design and method: In a single-step study, 72 patients with EH II-nd stage, 1–3 degrees of raising blood pressure, high and very high cardiovascular risk occurred. Patients’ gender consists of 29.16% (21) of men, 70.84% (51) of women. The average age of the patients is 59.87 ± 7.98 yr. The control group consisted of 48 healthy individuals, comparable in age (43.36 ± 7.1 yr.) and sexual division (62.5% for women, 37.5% for men). A study of the 825C > T polymorphism of the GNB3 gene has been performed by real-time BDP. Results: The genetic marker 825C > T of the GNB3 gene polymorphism (rs5443) is determined by replacing thymine (T) with cytosine (C) in the gene position with 825 DNA (12p13). Substitution of C with T in the GNB3 protein at position 825 of the amino acid sequence is accompanied by impaired replication of the 9th exon, which leads to the loss of a portion of the polypeptide chain, consisting of 41 amino acids. Genotypes division inpatient group with EH and in the observation group are as follows: the TT genotype is found in 8.33% (6) patients and 4.16% (2) of the control group, the Tt genotype in 41.66% (30) and 50% (24), respectively, the tt genotype is found in 50% (36) of patients and in 45.83% (22) of practically healthy patients (p < 0.05). The partition of polymorphic variants corresponded to the population balance of Hardy-Weinberg (x2 = 3.28, p > 0.05). The relative frequency of the wild C-allele and the mutational T-allele (PT = 0.24; PC = 0.71) does not differ between the research and control groups (x2 < 1.0; p > 0.05). The studied polymorphism of the GNB3 gene (5443) is not associated with an increased risk of EH [OR = 1.18; 95% CI: 0.56–2.46; p > 0.05]. Conclusions: The alleles and genotypes of 825C > T polymorphism of the GNB3 gene (rs5443) are not risk factors for the development of EH in the examined.