Abstract
Objective: The article is to investigate the relationship between the polymorphism of transcription factor 7-like 2 (TCF7L2) gene rs7903146 and type 2 diabetes mellitus with obesity in Chinese Han population. Methods: 216 patients with type 2 diabetes mellitus (T2DM), 92 males, 124 females, 194 normal controls, 89 males and 105 females. The polymorphism of TCF7L2 gene rs7903146 was measured by PCR-RFLP. PCR amplifier was used for amplification reaction, followed by polymorphism analysis of TCF7L2 gene: the reaction system was 20 μl, among which restriction endonuclease was 0.5 μl and PCR product was 7.5 μl. Restriction enzyme SspI (Fermentas) was used for PCR product and was bathed at 37°C for 16 h. The enzyme digestion products were detected and genotypes were identified by 2% agarose gel electrophoresis. The genotyping was repeated in 10% of the samples, and the genotyping rate was 100%. Height and weight were measured and BMI calculated. According to BMI, the experimental group was further divided into the obese group (n = 137, BMI > 25 kg/m2) and the normal body mass group (n = 79, BMI 2). Normal control group: BMI 2: FBG P P > 0.05). There was a significant difference in genotype frequency between the control group and the experimental group (χ2 = 17.382, P P > 0.05). Genotype frequency at BMI level was significantly different between the control group and the T2DM group (χ2 = 20.427, P = 0.001), but there was no significant difference in allele frequency distribution (P > 0.05). The TT type and TC + CC type were significantly different between the T2DM group and the control group (χ2 = 78.154, P = 0.006) and the T2DM obesity group and the control group (χ2 = 7.247, P = 0.009), but there was no significant difference between the T2DM normal body mass group and the control group (P > 0.05). In addition, there was no significant difference between TC type + TT type and CC type between the T2DM group and the control group and the T2DM obesity group and the control group (P > 0.05). Conclusion: There were significant differences in the genotype frequency and recessive inheritance pattern (TT/CC + TC) of rs7903146, suggesting that this SNP may be associated with the incidence of T2DM in obesity and insulin resistance.
Highlights
China is the second largest number of diabetics in the world with nearly 30 million [1]
Genotype frequency at body mass index (BMI) level was significantly different between the control group and the type 2 diabetes mellitus (T2DM) group (χ2 = 20.427, P = 0.001), but there was no significant difference in allele frequency distribution (P > 0.05)
The clinical data of FBG, 2 h postpranational BG, FIN and LgHOMA-IR were significantly different between the control group and the T2DM groups (P < 0.01), while the other parameters showed no statistical difference (P > 0.05)
Summary
China is the second largest number of diabetics in the world with nearly 30 million [1]. Previous study shows rs7903146 of transcription factor 7-like 2 (TCF7L2) is closely related to the occurrence of type 2 diabetes mellitus (T2DM) in different populations and nationalities, which plays a very important role [2]. Other studies have shown that this gene was associated with increasing body mass index (BMI), increasing LDL cholesterol (LDLC), decreasing triglycerides (RG), decreasing insulin secretion and obesity [3]. PCR-RFLP was used to investigate the relationship between rs7903146 polymorphism and type 2 diabetes with obesity in Chinese Han population [4]
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