Abstract
The blood samples were collected from dairy cows at the same milking stage. The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5'-flanking region of the hsp70 gene. The mRNA expression levels of HSP70,HSF1, Bcl-2 and Bax-α at different daily-mean-temperature were analyzed by relative quantitative RT- PCR. The DNA content, cell phase and the ratio of apoptosis of lymphocytes in peripheral blood of dairy cattle at different daily-mean- temperature were determined by FCM. The PCR-SSCP products of primer pair 1 showed polymorphisms and could be divided into four genotypes: aa, ab, ac, cc, with the cis-acting element (CCAAT box) included. Mutations in the hsp70 5'-flanking region (468-752 bp) had different effects on mRNA expression of HSP70, HSF1, Bcl-2 and Bax-α. The ac genotypic cows showed higher expressions of HSP70mRNA, HSF1mRNA and Bcl-2mRNA/Bax-αmRNA and lower ratio of apoptosis. These mutation sites can be used as molecular genetic markers to assist selection for anti-heat stress cows. (Asian-Aust. J. Anim. Sci. 2005. Vol 18, No. 5 : 734-740)
Highlights
Cows of apoptosis induced by the heat shock response is still suffer heat shock when the temperature is over 20°C, and unknown
Many studies show that the ratio of apoptosis can the milk yield decline significantly
A few of reports explored the protein participates in cell apoptosis and Ca2+ and p53 are relationship between genetic polymorphism of some genes thought to be involved in the regulation of the Bax protein and milk yield and quality (Chrenck et al, 2003; Badola et (Yic, 1997; Ross, 1998; Villunger, 2003; Perfettini, 2004). al., 2004)
Summary
Cows of apoptosis induced by the heat shock response is still suffer heat shock when the temperature is over 20°C, and unknown. Many studies show that the ratio of apoptosis can the milk yield decline significantly A few of reports explored the protein participates in cell apoptosis and Ca2+ and p53 are relationship between genetic polymorphism of some genes thought to be involved in the regulation of the Bax protein and milk yield and quality (Chrenck et al, 2003; Badola et (Yic, 1997; Ross, 1998; Villunger, 2003; Perfettini, 2004). Heat-shock 2mRNA/Bax-αmRNA of the lymphocytes in the peripheral proteins are bound to heat-shock factors (HSFs) within the blood of dairy cattle at different daily-mean-temperatures. Received July 17, 2004; Accepted November 12, 2004 for thermotolerance cows and improve the milk yield in
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