Polymorphism of The Promote Region of Chicken Insulin like Growth Factor-1 and Association With Bone Abnormalitiesالتغ

Abstract

The insulin like growth factor-1(IGF-1) is important regulators in stimulating growth, proteinsynthesis, cell proliferation and differentiation in a variety of cell types. To achieve this goal,blood samples were collected from 50 Gallus gallus affected from bone abnormalities (distortion)and 50 samples a control group from the Animal farm/ College of Veterinary medicine/University of Baghdad. DNA was isolated and the IGF-1genewas amplified by using specificprimers for promoter region and 5' UTR of this genes, then DNA sequencing was performed byusing AB13730XL, (Applied Bio system, Macro Gen company, USA). The DNA sequencingresults of flank sense of IGF-1gene from a control group was found to be compatible 100% withwild type of Gallus gallus from the Gene Bank. On the other hand, sequencing results of the samegene from 35cases abnormalities (distortion) revealed of 99% compatible and score 1351 andexpect 0.0 with the wild type sequences of gene bank. The minor differences could be attributedto two transversion substitutions of (G>T,T>G) and (C>T) on locations 404, 104, and 249 nucleicacid respectability. Fifteen cases of bone abnormalities (Distortion) of Gallus gallus, 99%compatibility have one Transversion substitution G>T on position 404 andone transitionsubstitution (C>T) on 249 positions, under number Sequence ID: gb|JX414253.1|. In conclusion,our case study suggests that polymorphisms of IGF-1gene are strongly associated with vertebrategrowth and development of some chicken breeds in Iraq. The aim of this study is to identify thegenetic polymorphisms of IGF-1gene and its association with vertebrate growth and developmentof some chicken breeds in Iraq.