Polymorphism of MMP1 and MMP3 promoter regions and HR-HPV infection in women from Burkina Faso and Côte d'Ivoire.

Bado Prosper,Ouedraogo Teega-Wendé Clarisse,Ouattara Abdoul Karim,Simpore Jacques,Ouedraogo Charlemagne,Bello Shoukrat Ohuwa Toyin,Yonli Albert Théophane,Traore Ina Marie Angèle,Horo Apollinaire,Obiri-Yeboah Dorcas,Setor Marius Ayaovi,Djigma Wendkuuni Florencia,Kouakou Kouame Privat,Traoré Esther Mah Alima,Zohoncon Théodora Mahoukèdè

doi:10.1515/bmc-2020-0010

Abstract

The single nucleotide polymorphism (SNP) of the promoter region of MMP-1 (at 1607 bp) and MMP-3 (at 1171 bp) create Ets binding sites. Correlations between these SNPs and sensitivity to several biological processes such as metastasis and recurrence of cancer have been reported in several studies. In this case-control study, we looked for these SNPs in women infected with or not with high-risk human papillomaviruses (HR-HPV). The frequency, distribution and correlation of these SNPs with the presence or absence of HR-HPV infection were evaluated. Genotypes 1G1G, 1G2G and 2G2G for MMP1 and genotypes 5A5A, 5A6A, 6A6A for MMP3 were found in our study population. In general, we noted that the 1G (40.8%) and 2G (64.8%) alleles were more frequent in non-infected women and infected women, respectively, and more specifically this difference was significant in women from Côte d'Ivoire. These results, although yet to be reaffirmed with assays for quantifying the mRNA of these genes, suggest that the SNP of the MMP-1 promoter could promote infection with HR-HPV.

Highlights

Cervix cancer is one of the most common female cancers in women worldwide, with more than 570,000 new cases and more than 311,000 deaths each year [1]
The objective of this study was to determine the associations between single nucleotide polymorphism (SNP) at positions 1607 and 1617 in MMP1 and MMP3 genes, respectively, and HR-PV infection
The swabs were collected in two countries in West Africa, namely Ivory Cost (IC) and Burkina Faso (BFA)

Summary

Introduction

Cervix cancer is one of the most common female cancers in women worldwide, with more than 570,000 new cases and more than 311,000 deaths each year [1]. It ranks second in terms of incidence and mortality behind breast cancer in context of low Human Development Index (HDI). HPVs infect skin and mucosal epithelial cells of the anogenital tract using several mechanisms, such as the attachment of the virus to several types of molecules in the extracellular matrix and its internalization by endocytosis. Laminins 5 (LN5) and Heparan sulphate proteoglycans (HSPG) are examples of the extracellular matrix (ECM) molecules which are used

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Conclusion