Abstract
A study was conducted to identify the polymorphism of keratin intermediate filament type-I (KIF-I) gene and its association with wool quality traits in Magra sheep. Blood samples and greasy fleece yield data of 162 adult males and females of Magra sheep (2 to 4 yr old) were collected. Following isolation of genomic DNA, genotyping of KIF-I gene was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The PCR-RFLP of KIF-I gene at locus KRT1.2 generated three genotype viz., AA, AB and BB with genotype frequencies of 0.49, 0.43 and 0.08, respectively. The frequencies for A and B allele were 0.71 and 0.29, respectively. Hardy-Weinberg equilibrium indicated that the population was in genetic equilibrium with respect to KIF-I gene. In conclusion, greater frequencies of allele A and genotype AA and AB are suggestive of its importance in quality of wool traits in Magra sheep.
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