Polymorphism of Insulin-induced Gene 1 (INSIG1) in Bali cattle (Bos javanicus) from small farmer at Badung district, Bali island

Abstract

Bali cattle is originated from Bali Island, Indonesia and domesticated from Banteng (Bibos banteng). Bali cattle are well known as a beef cattle with good performance, high carcass percentage and good reproduction traits. Carcass and growth traits were influenced by genetic and environment. Genetically, both of them was influenced several gene or polygene. Insulin- induced gene 1 (INSIG1) is one of the gene which effected of growth traits in cattle. The aim of this study was to identify the polymorphism of INSIG1 gene in Bali cattle from small farmer at Badung district, Bali. Fifty three of fresh bloods were taken from Bali cattle and DNA was extracted using high salt method. Detection of SNP A4366G in INSIG1 gene used PCR-RFLP with TaqI restriction enzyme. Data was analyzed by Cervus 3.07 including allele and genotype frequencies, heterozygosity while Hardy Weinberg equilibrium was calculated directly using formula. Results showed that three variants of genotype (AA, AG, and GG) with two alleles, A (0.5%) and G (0.5%) were found in this population and polymorphism was detected. Heterozygosity observed and expected were 0.505 and 0.500, respectively. PIC value reached 0.375. This population was in genetic equilibrium based on HWE. In conclusion, polymorphism of INSIG1 gene in SNP A4366G of Bali cattle from small farmer at Badung district was detected and has genetic diversity. This result could be early genetic information of Bali cattle to identify of their potencies.